4OFV

Refinement of RAGE-DNA complex in 3S58 without DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 61
Unit cell lengths	77.920, 77.920, 224.387
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.140 - 3.100
R-factor	0.221
Rwork	0.220
R-free	0.24000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3S58
RMSD bond length	0.002
RMSD bond angle	0.673
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.100
Number of reflections	13817
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	298	12% PEG6000, 0.1 M Tris-HCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K