4OFT
C- Orthorombic NaGST1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 48.630, 80.890, 200.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.140 - 2.600 |
| R-factor | 0.22968 |
| Rwork | 0.228 |
| R-free | 0.26999 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2on7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.097 |
| Data reduction software | CrysalisPro (Pro) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.260 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.061 | 0.639 |
| Number of reflections | 12651 | |
| <I/σ(I)> | 14.9 | 2.15 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 8.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 298 | 0.1 M sodium acetate, pH 4.6, 30% PEG 400, 100mM zinc protoporphyrin, VAPOR DIFFUSION, temperature 298K |
