4OFS
Crystal structure of a truncated catalytic core of the 2-oxoacid dehydrogenase multienzyme complex from Thermoplasma acidophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.151, 107.151, 238.618 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.870 - 4.100 |
| R-factor | 0.21566 |
| Rwork | 0.212 |
| R-free | 0.28430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rqc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.632 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.170 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.122 | 0.551 |
| Number of reflections | 12274 | |
| <I/σ(I)> | 13.06 | 2.48 |
| Completeness [%] | 99.2 | 97.4 |
| Redundancy | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 8% (w/v) PEG4K; 3% (w/v) PGA; 0.1M Na Cacodylate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
