4OFP
Crystal Structure of SYG-2 D3-D4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 121.240, 121.240, 178.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.315 - 3.000 |
R-factor | 0.2439 |
Rwork | 0.242 |
R-free | 0.28340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ofk |
RMSD bond length | 0.006 |
RMSD bond angle | 1.055 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1492)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 27351 |
<I/σ(I)> | 13.15 |
Completeness [%] | 99.9 |
Redundancy | 18.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 295 | 0.9 M Diammonium tartrate, 0.1 M Sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K |