4OFN
Monoclinic NaGST1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Detector technology | CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.554, 45.213, 113.763 |
| Unit cell angles | 90.00, 112.11, 90.00 |
Refinement procedure
| Resolution | 14.850 - 2.690 |
| R-factor | 0.29436 |
| Rwork | 0.292 |
| R-free | 0.33036 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2on7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.971 |
| Data reduction software | CrysalisPro (Pro) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.400 | 2.850 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.095 | |
| Number of reflections | 10767 | |
| <I/σ(I)> | 8.3 | 4.4 |
| Completeness [%] | 97.1 | 97.7 |
| Redundancy | 2.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1 M sodium acetate, pH 4.6, 30% PEG 400, 100mM protoporphyrin IX disodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
