4OFM
Triclinic NaGST1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.197, 47.839, 103.116 |
| Unit cell angles | 80.54, 78.70, 61.99 |
Refinement procedure
| Resolution | 14.750 - 2.640 |
| R-factor | 0.22177 |
| Rwork | 0.219 |
| R-free | 0.26916 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2on7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.608 |
| Data reduction software | CrysalisPro (Pro) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.760 | 2.850 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.068 | 0.088 |
| Number of reflections | 19062 | |
| <I/σ(I)> | 7.7 | 3.7 |
| Completeness [%] | 84.8 | 86.5 |
| Redundancy | 1.6 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1 M sodium acetate, pH 4.6, 30% PEG 400, 100mM protoporphyrin IX disodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
