4OF0
Crystal Structure of SYG-1 D1-D2, refolded
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9796,0.9797,0.9719 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.545, 32.065, 102.235 |
| Unit cell angles | 90.00, 113.84, 90.00 |
Refinement procedure
| Resolution | 46.756 - 2.300 |
| R-factor | 0.2053 |
| Rwork | 0.201 |
| R-free | 0.24540 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.808 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1160)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 11098 |
| <I/σ(I)> | 19.3 |
| Completeness [%] | 90.7 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 20% PEG 3,350, 0.2 M Ammonium acetate, 0.1 M HEPES pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K |
