4OEZ

Crystal structure of AR-LBD bound with co-regulator peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	150
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	55.892, 66.267, 70.541
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.440 - 1.800
R-factor	0.224
Rwork	0.222
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oea
RMSD bond length	0.019
RMSD bond angle	1.917
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.800
Rmerge	0.039
Number of reflections	24972
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.6 M MAGNESIUM SULFATE HEPTAHYDRATE IN 0.1 MM MES BUFFER, PH 6.5 , VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.6 M MAGNESIUM SULFATE HEPTAHYDRATE IN 0.1 MM MES BUFFER, PH 6.5 , VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K