4OEN
Crystal structure of the second substrate binding domain of a putative amino acid ABC transporter from Streptococcus pneumoniae Canada MDR_19A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.432, 109.606, 57.922 |
| Unit cell angles | 90.00, 111.11, 90.00 |
Refinement procedure
| Resolution | 27.401 - 1.650 |
| R-factor | 0.1626 |
| Rwork | 0.161 |
| R-free | 0.19610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wdn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine: dev_1538)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 58402 | |
| <I/σ(I)> | 10.85 | 3.13 |
| Completeness [%] | 98.8 | 98.9 |
| Redundancy | 4.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 2.5 M ammonium sulfate, 0.1 M sodium acetate pH4.6, 2% Hexandiol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
