4OEB

Structure of membrane binding protein pleurotolysin A from Pleurotus ostreatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-16
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95364
Spacegroup name	P 62
Unit cell lengths	131.700, 131.700, 65.213
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.109 - 1.850
R-factor	0.1737
Rwork	0.172
R-free	0.19820
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.109	2.110
High resolution limit [Å]	1.850	1.850
Rmerge	0.113	0.561
Number of reflections	54873
<I/σ(I)>	49.9	11.5
Completeness [%]	100.0	100
Redundancy	88.1	87.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	20 mM sodium cacodylate, pH 6.0, 14% PEG3350, 0.2 M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K