4OE1

Crystal structure of the pentatricopeptide repeat protein PPR10 (C256S/C430S/C449S) in complex with an 18-nt PSAJ rna element

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 43
Unit cell lengths	83.318, 83.318, 225.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.259 - 2.800
R-factor	0.2636
Rwork	0.263
R-free	0.27760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m59
RMSD bond length	0.015
RMSD bond angle	0.999
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	37060
Completeness [%]	98.0	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	9% PEG 3350, 0.1M MES 5.5, 6% Tacsimate, pH 6.0, 70 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 291K