4ODW
Unliganded Fab structure of lipid A-specific antibody A6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 |
Unit cell lengths | 42.129, 69.235, 69.066 |
Unit cell angles | 70.99, 72.39, 88.29 |
Refinement procedure
Resolution | 65.270 - 2.720 |
R-factor | 0.2325 |
Rwork | 0.231 |
R-free | 0.25660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4odv |
RMSD bond length | 0.011 |
RMSD bond angle | 1.613 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 65.270 | 35.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.040 | 0.031 | 0.472 |
Number of reflections | 18489 | ||
<I/σ(I)> | 15.4 | ||
Completeness [%] | 98.1 | 97.1 | 98.3 |
Redundancy | 2 | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Tris-HCl, 35% (w/v) PEG 1000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |