4ODK
Structure of SlyD from Thermus thermophilus in complex with T1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9191 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.500, 52.990, 48.910 |
| Unit cell angles | 90.00, 111.18, 90.00 |
Refinement procedure
| Resolution | 29.096 - 1.401 |
| R-factor | 0.1403 |
| Rwork | 0.139 |
| R-free | 0.16710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.027 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.100 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 40188 | |
| <I/σ(I)> | 23.03 | 2.02 |
| Completeness [%] | 97.6 | 93.7 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 13.2% PEG1500, 0.1 M HEPES, pH 7.5, 0.05 M sodium chloride, 11% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
