4ODB
Crystal structure of the T1L reovirus attachment protein sigma1 in complex with Junctional Adhesion Molecule-A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 156.770, 156.770, 96.540 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.980 - 3.200 |
R-factor | 0.2141 |
Rwork | 0.211 |
R-free | 0.24250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3EOY (modified with chainsaw) |
RMSD bond length | 0.009 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.280 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.074 | 0.376 |
Number of reflections | 22673 | |
<I/σ(I)> | 14.8 | 3.3 |
Completeness [%] | 99.1 | 99.5 |
Redundancy | 4.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293.15 | 0.1 M MES, 17.1% PEG 20000, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |