4OCR
Crystal structure of human Fab CAP256-VRC26.01, a potent V1V2-directed HIV-1 neutralizing antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 104.398, 71.165, 82.880 |
Unit cell angles | 90.00, 93.34, 90.00 |
Refinement procedure
Resolution | 34.365 - 1.895 |
R-factor | 0.1812 |
Rwork | 0.180 |
R-free | 0.19960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u4e |
RMSD bond length | 0.008 |
RMSD bond angle | 1.259 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.895 | 5.160 | 1.895 |
Rmerge | 0.077 | 0.035 | 0.537 |
Number of reflections | 48063 | ||
<I/σ(I)> | 16.3 | 32.7 | 2.1 |
Completeness [%] | 100.0 | 99.5 | 99.8 |
Redundancy | 3.7 | 3.7 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 32% PEG400, 4% PEG3350, 0.1 M sodium acetate, pH 5.5, cryoprotectant: 20% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |