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4OCM

Crystal Structure of the Rpn8-Rpn11 MPN domain heterodimer, crystal form Ib

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID23-1
Synchrotron siteESRF
BeamlineID23-1
Temperature [K]100
Detector technologyCCD
Collection date2013-10-27
DetectorADSC QUANTUM 315r
Wavelength(s)1.00000
Spacegroup nameP 1 2 1
Unit cell lengths63.401, 44.970, 200.043
Unit cell angles90.00, 98.40, 90.00
Refinement procedure
Resolution30.000 - 1.990
R-factor0.2181
Rwork0.216
R-free0.26230
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4ocl
RMSD bond length0.022
RMSD bond angle1.933
Data reduction softwareXDS
Data scaling softwareSCALA (3.3.16)
Phasing softwareMOLREP
Refinement softwareREFMAC (5.5.0109)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]197.89844.9702.100
High resolution limit [Å]1.9906.2901.990
Rmerge0.0780.0300.600
Total number of observations771731869
Number of reflections74800
<I/σ(I)>9.116.41.3
Completeness [%]96.897.988
Redundancy3.233.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROPS629150 mM MES, pH 6.0, 200 mM calcium acetate, 22% PEG3350, VAPOR DIFFUSION, HANGING DROPS, temperature 291K

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