4OCM
Crystal Structure of the Rpn8-Rpn11 MPN domain heterodimer, crystal form Ib
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 63.401, 44.970, 200.043 |
Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.990 |
R-factor | 0.2181 |
Rwork | 0.216 |
R-free | 0.26230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ocl |
RMSD bond length | 0.022 |
RMSD bond angle | 1.933 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.16) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 197.898 | 44.970 | 2.100 |
High resolution limit [Å] | 1.990 | 6.290 | 1.990 |
Rmerge | 0.078 | 0.030 | 0.600 |
Total number of observations | 7717 | 31869 | |
Number of reflections | 74800 | ||
<I/σ(I)> | 9.1 | 16.4 | 1.3 |
Completeness [%] | 96.8 | 97.9 | 88 |
Redundancy | 3.2 | 3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROPS | 6 | 291 | 50 mM MES, pH 6.0, 200 mM calcium acetate, 22% PEG3350, VAPOR DIFFUSION, HANGING DROPS, temperature 291K |