4OCI
Crystal Structure of Calcium Binding Protein-5 from Entamoeba histolytica and its involvement in initiation of phagocytosis of human erythrocytes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77.15 |
| Detector technology | CCD |
| Collection date | 2011-11-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97887 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.552, 44.446, 47.730 |
| Unit cell angles | 90.00, 108.92, 90.00 |
Refinement procedure
| Resolution | 45.150 - 2.009 |
| R-factor | 0.18122 |
| Rwork | 0.179 |
| R-free | 0.22776 |
| Structure solution method | SAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.990 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.075 |
| Number of reflections | 9379 |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 2.8 M Sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
