4OCB
Z-DNA dodecamer d(CGCGCGCGCGCG)2 at 0.75 A resolution solved by P-SAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Detector technology | PIXEL |
Collection date | 2013-03-28 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.6199 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 48.484, 19.546, 31.224 |
Unit cell angles | 90.00, 116.36, 90.00 |
Refinement procedure
Resolution | 30.000 - 0.750 |
R-factor | 0.122 |
Rwork | 0.122 |
R-free | 0.13400 |
Structure solution method | SAD |
RMSD bond length | 0.021 |
RMSD bond angle | 0.050 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 0.760 |
High resolution limit [Å] | 0.750 | 0.750 |
Rmerge | 0.031 | 0.734 |
Number of reflections | 32210 | |
<I/σ(I)> | 84.7 | 2.8 |
Completeness [%] | 95.1 | 92.2 |
Redundancy | 13.5 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2:1 mixture of 1.66 mM DNA solution with the following solution: 40mM Na cacodylate pH 7.0, 12 mM spermine tetrachloride, 80 mM NaCl, 10% (v/v) MPD. The well solution contained 35% MPD., VAPOR DIFFUSION, HANGING DROP, temperature 293K |