4OCA

Cryatal structure of ArnB K188A complexted with PLP and UDP-Ara4N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	113
Collection date	2013-01-19
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	90.952, 90.952, 129.123
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.179 - 2.300
R-factor	0.2281
Rwork	0.226
R-free	0.26080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.692
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.180	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	29611

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	0.1M Sodium Citrate (pH4.5), 19 % PEG 20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K