4OC8
DNA modification-dependent restriction endonuclease AspBHI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.06296 |
| Spacegroup name | P 62 |
| Unit cell lengths | 195.092, 195.092, 81.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.739 - 2.884 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23520 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.869 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.8_1069) |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.740 | 2.990 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.116 | 0.820 |
| Number of reflections | 40065 | |
| <I/σ(I)> | 19.7 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.9 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 289 | 12% polyethylene glycol 3350, 0.5 M K2HPO4/Na2HPO4, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
