4OBM

Crystal structure of a putative transcription regulator (EUBSIR_01389) from Eubacterium siraeum DSM 15702 at 2.15 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91837,0.97953,0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	49.503, 51.466, 104.712
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.189 - 2.150
R-factor	0.2044
Rwork	0.202
R-free	0.24530
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.189		2.230
High resolution limit [Å]	2.150	4.610	2.150
Rmerge	0.100	0.055	0.510
Number of reflections	14819	1593	1483
<I/σ(I)>	7.53	15	2.2
Completeness [%]	97.7	94.9	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6.5	277	40.0000% 2-methyl-2,4-pentanediol, 5.0000% polyethylene glycol 8000, 0.1M sodium cacodylate pH 6.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K