4OBI

Crystal structure of a DUF1312 family protein (EF3258) from Enterococcus faecalis V583 at 1.73 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-13
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	27.259, 49.160, 71.776
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.559 - 1.734
R-factor	0.1704
Rwork	0.169
R-free	0.19920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.091
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.559		1.790
High resolution limit [Å]	1.730	3.720	1.730
Rmerge	0.054	0.024	0.684
Number of reflections	10218	1143	794
<I/σ(I)>	15.25	44.4	1.9
Completeness [%]	96.4	96.1	78.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6	293	30.0000% polyethylene glycol 6000, 0.1M MES pH 6.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K