4OBE

Crystal Structure of GDP-bound Human KRas

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97926
Spacegroup name	C 1 2 1
Unit cell lengths	66.147, 42.096, 114.393
Unit cell angles	90.00, 105.32, 90.00

Refinement procedure

Resolution	22.065 - 1.240
R-factor	0.1572
Rwork	0.157
R-free	0.16860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4epv
RMSD bond length	0.010
RMSD bond angle	1.427
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1523))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.260
High resolution limit [Å]	1.240	3.370	1.240
Rmerge	0.049	0.029	0.395
Number of reflections	81203
<I/σ(I)>	2.06	56.6	2.1
Completeness [%]	100.0	96.5	94.1
Redundancy	4.3	4.7	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2M Sodium Acetate pH4.5, 0.1M Tris pH 8.5, 26% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K