4OAY
BldD CTD-c-di-GMP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-01 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 32 |
Unit cell lengths | 86.500, 86.500, 151.480 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 74.910 - 1.950 |
R-factor | 0.208 |
Rwork | 0.198 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | P32 STARTING MODEL WITH SEMET |
RMSD bond length | 0.021 |
RMSD bond angle | 2.512 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.700 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.057 | 0.395 |
Number of reflections | 90983 | |
<I/σ(I)> | 11.4 | 1.7 |
Completeness [%] | 98.5 | 62.2 |
Redundancy | 3.5 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 298 | 35% PEG 400, 0.1 M acetic acid pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |