4OAW
Fab structure of anti-HIV gp120 V2 mAb 2158
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-11-27 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 32 |
Unit cell lengths | 73.710, 73.710, 215.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.831 - 2.800 |
R-factor | 0.1859 |
Rwork | 0.182 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qad |
RMSD bond length | 0.010 |
RMSD bond angle | 1.338 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 215.000 | 2.800 |
High resolution limit [Å] | 2.800 | 2.760 |
Number of reflections | 31988 | |
<I/σ(I)> | 5.6 | 1.8 |
Completeness [%] | 98.2 | 98.5 |
Redundancy | 5.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 23.5% PEG 4K/0.17M ammonium sulfate/15% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |