4OAI
Crystal structure of the cytosolic domain of mouse MiD51 dimer mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.682, 67.101, 79.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.550 - 2.000 |
R-factor | 0.1816 |
Rwork | 0.179 |
R-free | 0.22340 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.134 |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.550 | 33.550 | 1.960 |
High resolution limit [Å] | 1.857 | 5.870 | 1.860 |
Rmerge | 0.012 | 0.012 | |
Number of reflections | 28905 | ||
<I/σ(I)> | 14.1 | 29.2 | 0.7 |
Completeness [%] | 98.4 | 98.3 | 91.4 |
Redundancy | 4.8 | 4.6 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296.15 | 200 mM lithium sulfate, 20% PEG 3350, hanging drop, temperature 296.15K, VAPOR DIFFUSION, HANGING DROP |