4OAI

Crystal structure of the cytosolic domain of mouse MiD51 dimer mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03
Spacegroup name	P 21 21 21
Unit cell lengths	63.682, 67.101, 79.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.550 - 2.000
R-factor	0.1816
Rwork	0.179
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.134
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.550	33.550	1.960
High resolution limit [Å]	1.857	5.870	1.860
Rmerge		0.012	0.012
Number of reflections	28905
<I/σ(I)>	14.1	29.2	0.7
Completeness [%]	98.4	98.3	91.4
Redundancy	4.8	4.6	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296.15	200 mM lithium sulfate, 20% PEG 3350, hanging drop, temperature 296.15K, VAPOR DIFFUSION, HANGING DROP