4OAH
Crystal structure of the cytosolic domain of mouse MiD51 H201A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.434, 79.151, 103.449 |
| Unit cell angles | 90.00, 98.04, 90.00 |
Refinement procedure
| Resolution | 31.506 - 2.000 |
| R-factor | 0.1728 |
| Rwork | 0.172 |
| R-free | 0.21690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.205 |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.580 | 36.326 | 1.950 |
| High resolution limit [Å] | 1.850 | 5.860 | 1.850 |
| Rmerge | 0.024 | 0.978 | |
| Number of reflections | 106662 | ||
| <I/σ(I)> | 20.3 | 0.8 | |
| Completeness [%] | 95.5 | 97.1 | 80.1 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296.15 | 100 mM Bis-Tris pH 6.5, 200 mM lithium sulfate, 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 296.15K |
