4OAH
Crystal structure of the cytosolic domain of mouse MiD51 H201A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.434, 79.151, 103.449 |
Unit cell angles | 90.00, 98.04, 90.00 |
Refinement procedure
Resolution | 31.506 - 2.000 |
R-factor | 0.1728 |
Rwork | 0.172 |
R-free | 0.21690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.205 |
Data scaling software | SCALA |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.580 | 36.326 | 1.950 |
High resolution limit [Å] | 1.850 | 5.860 | 1.850 |
Rmerge | 0.024 | 0.978 | |
Number of reflections | 106662 | ||
<I/σ(I)> | 20.3 | 0.8 | |
Completeness [%] | 95.5 | 97.1 | 80.1 |
Redundancy | 3.2 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296.15 | 100 mM Bis-Tris pH 6.5, 200 mM lithium sulfate, 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 296.15K |