4OAG
Crystal structure of the cytosolic domain of mouse MiD51 bound to ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-01-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.01 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.068, 80.811, 65.376 |
Unit cell angles | 90.00, 105.74, 90.00 |
Refinement procedure
Resolution | 33.999 - 2.000 |
R-factor | 0.1731 |
Rwork | 0.171 |
R-free | 0.21620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.148 |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.705 | 34.705 | 2.040 |
High resolution limit [Å] | 1.939 | 6.130 | 1.940 |
Rmerge | 0.018 | 0.442 | |
Number of reflections | 44016 | ||
<I/σ(I)> | 15.6 | 33.2 | 1.8 |
Completeness [%] | 95.6 | 97.5 | 82.2 |
Redundancy | 3.4 | 3.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296.15 | 100 mM Bis-Tris, 100 mM lithium sulfate, 10 mM magnesiun chloride, 25% PEG 3350, 10 mM ADP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.15K |