4OAF
Crystal structure of the cytosolic domain of mouse MiD51
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.075, 78.592, 102.310 |
Unit cell angles | 90.00, 96.61, 90.00 |
Refinement procedure
Resolution | 32.005 - 2.200 |
R-factor | 0.2054 |
Rwork | 0.204 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.141 |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.296 | 39.296 | 1.970 |
High resolution limit [Å] | 1.872 | 5.920 | 1.870 |
Rmerge | 0.021 | 0.021 | |
Number of reflections | 110262 | ||
<I/σ(I)> | 6.9 | 18.9 | 0.4 |
Completeness [%] | 93.4 | 93.2 | 74.8 |
Redundancy | 2.8 | 2.9 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296.15 | 100 mM HEPE, 700 mM Potassium sodium tartrate tetrahydrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.15K |