4OAA
Crystal structure of E. coli lactose permease G46W,G262W bound to sugar
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.115869 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 101.560, 121.940, 264.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.000 - 3.500 |
R-factor | 0.2583 |
Rwork | 0.257 |
R-free | 0.29290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cfq |
RMSD bond length | 0.006 |
RMSD bond angle | 0.899 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 3.200 |
High resolution limit [Å] | 2.980 | 2.980 |
Rmerge | 0.110 | 0.194 |
Number of reflections | 31737 | |
<I/σ(I)> | 6.47 | 0.08 |
Completeness [%] | 95.3 | 61.7 |
Redundancy | 3.8 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 10mg/ml protein (20mM Hepes, .2% NG)and 5mM TDG against 1M NaCl, 0.05M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |