Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

4OA8

X-ray crystal structure of O-methyltransferase from Anaplasma phagocytophilum in apo form

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-G
Synchrotron siteAPS
Beamline21-ID-G
Temperature [K]100
Detector technologyCCD
Collection date2013-06-05
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)0.9786
Spacegroup nameP 21 2 21
Unit cell lengths73.150, 76.400, 79.530
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution44.050 - 2.150
R-factor0.1872
Rwork0.185
R-free0.23180
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4oa5
RMSD bond length0.015
RMSD bond angle1.597
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwareREFMAC (5.7.0032)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]50.0002.210
High resolution limit [Å]2.1509.6202.150
Rmerge0.0480.0120.491
Number of reflections244843011798
<I/σ(I)>27.4990.813.39
Completeness [%]98.288.598.1
Redundancy4.71
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP8.52890.4 uL protein @ 20 mg/mL + 0.4 uL JCSG+ well D6 - 0.2 M magnesium chloride, 0.1 M TRIS pH 8.50, 20% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 289K

222624

PDB entries from 2024-07-17

PDB statisticsPDBj update infoContact PDBjnumon