4O9T
Mechanism of transhydrogenase coupling proton translocation and hydride transfer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.221, 86.388, 125.149 |
| Unit cell angles | 90.00, 93.99, 90.00 |
Refinement procedure
| Resolution | 46.300 - 3.079 |
| R-factor | 0.22249 |
| Rwork | 0.219 |
| R-free | 0.27963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.548 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.300 |
| High resolution limit [Å] | 3.079 |
| Number of reflections | 36118 |
| Completeness [%] | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | lipidic subic phase | 8.5 | 298 | 350 mM NH4-formate, 100mM Na-thiocynate, and 18% (v/v) 1-4-butanediol, pH 8.5, lipidic subic phase, temperature 298.0K |
