4O9D
Structure of Dos1 propeller
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-20 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.150, 102.790, 95.100 |
| Unit cell angles | 90.00, 122.36, 90.00 |
Refinement procedure
| Resolution | 45.870 - 2.000 |
| R-factor | 0.2142 |
| Rwork | 0.213 |
| R-free | 0.25010 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.209 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.870 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.346 |
| Number of reflections | 65983 | |
| <I/σ(I)> | 11.9 | 3.05 |
| Completeness [%] | 99.0 | 99.5 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 291.15 | 50mM Tris-HCl pH=7.0, 0.9M di-sodium tartrate, 50mM Magnesium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
