4O95
Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (ZmCKO4) in complex with phenylurea inhibitor CPPU
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-08 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97934 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.450, 74.450, 208.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.670 - 1.750 |
R-factor | 0.188 |
Rwork | 0.187 |
R-free | 0.20710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 58646 | |
<I/σ(I)> | 13.14 | |
Completeness [%] | 97.5 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 22% ethylene glycol, co-crystallized with 3mM CPPU in DMSO, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |