4O94
Crystal structure of a trap periplasmic solute binding protein from Rhodopseudomonas palustris HaA2 (RPB_3329), Target EFI-510223, with bound succinate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-26 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.469, 112.845, 136.294 |
Unit cell angles | 90.00, 95.91, 90.00 |
Refinement procedure
Resolution | 45.190 - 2.000 |
R-factor | 0.1657 |
Rwork | 0.163 |
R-free | 0.21440 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.316 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHENIX (AUOTSOLVE) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 135.570 | 135.570 | 2.110 |
High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
Rmerge | 0.120 | 0.052 | 0.610 |
Total number of observations | 21857 | 51688 | |
Number of reflections | 90352 | ||
<I/σ(I)> | 10.8 | 12.5 | 1.1 |
Completeness [%] | 95.8 | 99.6 | 86.5 |
Redundancy | 5.8 | 7.1 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop vapor diffusion | 4 | 298 | Protein (30.3 mg/ml, 10 mM HEPES pH 7.5, 5 mM DTT, 10 mM Succinate); Reservoir (1 M Lithium Chloride 0.1 M tri-Sodium Citrate pH 4, 20 %(w/v) PEG 6000); Cryoprotection (80% of 50% Peg3350, 20% Reservoir), sitting drop vapor diffusion, temperature 298K |