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4O94

Crystal structure of a trap periplasmic solute binding protein from Rhodopseudomonas palustris HaA2 (RPB_3329), Target EFI-510223, with bound succinate

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 31-ID
Synchrotron siteAPS
Beamline31-ID
Temperature [K]100
Detector technologyCCD
Collection date2013-11-26
DetectorRAYONIX MX225HE
Wavelength(s)0.9793
Spacegroup nameP 1 21 1
Unit cell lengths46.469, 112.845, 136.294
Unit cell angles90.00, 95.91, 90.00
Refinement procedure
Resolution45.190 - 2.000
R-factor0.1657
Rwork0.163
R-free0.21440
Structure solution methodSAD
RMSD bond length0.013
RMSD bond angle1.316
Data reduction softwareMOSFLM
Data scaling softwareSCALA (3.3.20)
Phasing softwarePHENIX (AUOTSOLVE)
Refinement softwarePHENIX (1.8.1_1168)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]135.570135.5702.110
High resolution limit [Å]2.0006.3202.000
Rmerge0.1200.0520.610
Total number of observations2185751688
Number of reflections90352
<I/σ(I)>10.812.51.1
Completeness [%]95.899.686.5
Redundancy5.87.14.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1sitting drop vapor diffusion4298Protein (30.3 mg/ml, 10 mM HEPES pH 7.5, 5 mM DTT, 10 mM Succinate); Reservoir (1 M Lithium Chloride 0.1 M tri-Sodium Citrate pH 4, 20 %(w/v) PEG 6000); Cryoprotection (80% of 50% Peg3350, 20% Reservoir), sitting drop vapor diffusion, temperature 298K

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