4O90
Crystal structure of chorismate synthase from Acinetobacter baumannii at 2.6A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-10-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 108.739, 73.192, 90.118 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.370 - 2.610 |
R-factor | 0.243 |
Rwork | 0.243 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lj2 |
RMSD bond length | 0.020 |
RMSD bond angle | 2.900 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.370 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 21001 | |
Completeness [%] | 94.0 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 50mM Tris-HCl, 0.2M MgCl2, 20% PEG3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |