4O8Q
Crystal structure of bovine MHD domain of the COPI delta subunit at 2.15 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-28 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.092, 110.333, 145.794 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.890 - 2.150 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.22900 |
Structure solution method | MR-SAD |
Starting model (for MR) | FROM INITIAL SAD EXPERIMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.159 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER ((phenix)) |
Refinement software | PHENIX ((phenix.refine: dev_1565)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.990 |
High resolution limit [Å] | 1.960 | 1.960 |
Number of reflections | 43300 | |
<I/σ(I)> | 28.2 | 2.7 |
Completeness [%] | 88.0 | 77.9 |
Redundancy | 2.8 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 293 | 0.2M Sodium citrate tribasic dehydrate, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||
2 | 293 | 0.4M NaCl, 0.1M Hepes 7.5, 18% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |