4O8O
Crystal structure of SthAraf62A, a GH62 family alpha-L-arabinofuranosidase from Streptomyces thermoviolaceus, bound to alpha-L-arabinose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.050, 65.739, 85.203 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.465 - 1.210 |
| R-factor | 0.1392 |
| Rwork | 0.139 |
| R-free | 0.15570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.384 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_1538)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.200 |
| High resolution limit [Å] | 1.178 | 1.178 |
| Number of reflections | 109436 | |
| <I/σ(I)> | 31.45 | 3.15 |
| Completeness [%] | 98.1 | 96.1 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 14 mg/mL SthAraf62A, 0.1 M HEPES pH 7.5, 0.2 M ammonium acetate, 25% PEG 3350, 200 mM alpha-L-arabinose, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
