4O8G
Structure of Infrared Fluorescent Protein 1.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.6 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.162, 53.228, 66.796 |
| Unit cell angles | 90.00, 90.60, 90.00 |
Refinement procedure
| Resolution | 24.040 - 1.652 |
| R-factor | 0.18606 |
| Rwork | 0.185 |
| R-free | 0.21073 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s7n |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.508 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.700 |
| High resolution limit [Å] | 1.620 | 1.650 |
| Number of reflections | 38000 | |
| <I/σ(I)> | 29.7 | 8.84 |
| Completeness [%] | 93.7 | 99.9 |
| Redundancy | 7.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | Mother liquor 6% PEG 4000, 10% glycerol, 0.07 M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
