4O88
Crystal structure of a C-tagged Nuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 130 |
Detector technology | PIXEL |
Collection date | 2013-01-24 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.176 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.879, 77.455, 147.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.950 - 2.900 |
R-factor | 0.1901 |
Rwork | 0.188 |
R-free | 0.24660 |
Structure solution method | Molecular refinement |
RMSD bond length | 0.005 |
RMSD bond angle | 1.065 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.950 | 3.070 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 16216 | |
Completeness [%] | 98.8 | 94.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 100 mM Sodium citrate, 3 - 3.5 M Ammonium sulfate, 100 mM Magnesium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |