4O7Z
SAICAR synthetase (Type-2) in complex with GMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-09-27 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 44.090, 155.180, 78.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.830 - 2.300 |
R-factor | 0.2168 |
Rwork | 0.214 |
R-free | 0.26680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u55 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.401 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.410 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.082 | 0.183 |
Number of reflections | 12282 | |
<I/σ(I)> | 10 | 5.2 |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 4.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch Underoil | 4.6 | 293 | 0.2M Ammonium Sulphate, 0.1M Sodium acetate trihydrate, 30% w/v PEG monomethyl ether 2000, pH 4.6, Microbatch Underoil, temperature 293K |