4O7Q
Crystal Structure of the F27G AIM2 Pyrin Domain Mutant and Similarities of its Self-association to DED/DED Interactions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-11 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 29.940, 37.950, 76.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.902 - 1.820 |
R-factor | 0.1805 |
Rwork | 0.178 |
R-free | 0.23780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | combined model |
RMSD bond length | 0.006 |
RMSD bond angle | 0.828 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.000 | 1.870 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.057 | 0.670 |
Number of reflections | 7526 | |
<I/σ(I)> | 29.7 | 3.1 |
Completeness [%] | 90.0 | 51 |
Redundancy | 10.6 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 289 | 0.2 M CaCl2, 0.1 M Sodium Acetate, and 23 % PEG3350, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |