4O7N
SAICAR synthetase (Type-2) in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-01-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 44.100, 154.500, 78.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.030 - 2.160 |
R-factor | 0.20404 |
Rwork | 0.202 |
R-free | 0.24820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u55 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.493 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.030 | 2.280 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.061 | 0.216 |
Number of reflections | 14847 | |
<I/σ(I)> | 15.8 | 6.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch Underoil | 4.6 | 293 | 0.2M Ammonium Sulphate, 0.1M Sodium acetate trihydrate, 30% w/v PEG monomethyl ether 2000, pH 4.6, Microbatch Underoil, temperature 293K |