4O6P
Structural and functional studies the characterization of C58G/C70G mutant in Cys4 Zinc-finger motif in the recombination mediator protein RecR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.005 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.862, 65.754, 91.755 |
| Unit cell angles | 90.00, 95.41, 90.00 |
Refinement procedure
| Resolution | 19.840 - 3.000 |
| R-factor | 0.236 |
| Rwork | 0.235 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vdp |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.858 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 10641 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.15 | 293 | 12%(W/V) PEG33500, 100MM SODIUM ACETATE, 2%(W/V) TACSIMATE, pH 5.15, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
