4O6G

Rv3902c from M. tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Detector technology	CCD
Collection date	2013-04-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	91.819, 91.819, 54.231
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.759 - 1.550
R-factor	0.166
Rwork	0.165
R-free	0.17670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Bromide Soaked SAD Crystal Structure
RMSD bond length	0.006
RMSD bond angle	0.968
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX (1.8.2_1309)
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.760
High resolution limit [Å]	1.550
Number of reflections	37938
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Protein Concentration 25mg/ml, 1.5M ammonium sulphate, and 200mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K