4O6C
West Nile Virus Non-structural protein 1 (NS1) Form 2 crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-12 |
Detector | MARMOSAIC 300 |
Wavelength(s) | 1.0332 |
Spacegroup name | P 3 |
Unit cell lengths | 186.885, 186.885, 81.765 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.981 - 2.751 |
R-factor | 0.2167 |
Rwork | 0.215 |
R-free | 0.23210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o6d |
RMSD bond length | 0.004 |
RMSD bond angle | 0.824 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.244 | 2.410 |
Number of reflections | 82996 | |
<I/σ(I)> | 7.4 | 1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.9 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 278 | 25% PEG 3000, 5% Glycerol, 20 mM Sodium Citrate pH 5.5, vapor diffusion, sitting drop, temperature 278K |