4O64
Zinc fingers of KDM2B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.28315 |
| Spacegroup name | P 31 |
| Unit cell lengths | 107.322, 107.322, 34.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.130 |
| R-factor | 0.1914 |
| Rwork | 0.191 |
| R-free | 0.21980 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.388 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.29) |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.470 | 46.470 | 2.190 |
| High resolution limit [Å] | 2.130 | 9.040 | 2.130 |
| Rmerge | 0.152 | 0.011 | 1.081 |
| Total number of observations | 1874 | 11282 | |
| Number of reflections | 24576 | ||
| <I/σ(I)> | 12.5 | 48.3 | 1.7 |
| Completeness [%] | 100.0 | 99.3 | 100 |
| Redundancy | 5.7 | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 8.5 | 291 | 25% PEG 8000, 0.2 M sodium chloride, 0.1 M tris, pH 8.5, sitting drop, temperature 291K |
