4O62
CW-type zinc finger of ZCWPW2 in complex with the amino terminus of histone H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.28303 |
| Spacegroup name | H 3 |
| Unit cell lengths | 127.095, 127.095, 63.046 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.460 - 1.780 |
| R-factor | 0.1656 |
| Rwork | 0.165 |
| R-free | 0.18600 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.370 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.14) |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.460 | 1.820 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.070 | 0.011 |
| Total number of observations | 11373 | |
| Number of reflections | 36371 | |
| <I/σ(I)> | 20.1 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 2 M ammonium sulfate, 2% PEG400, 0.1 M sodium HEPES, pH 7.5, vapor diffusion, temperature 291K |
