4O5R
Crystal structure of Alkylhydroperoxide Reductase subunit C from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-25 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 137.349, 137.349, 147.450 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.740 - 3.330 |
R-factor | 0.241 |
Rwork | 0.241 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3emp |
RMSD bond length | 0.004 |
RMSD bond angle | 0.696 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 147.450 | 3.510 |
High resolution limit [Å] | 3.330 | 3.330 |
Rmerge | 0.484 | |
Number of reflections | 23735 | |
<I/σ(I)> | 10.2 | 1.5 |
Completeness [%] | 99.2 | 99.7 |
Redundancy | 3.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.8M ammonium sulfate, 100mM MES, pH 6.5, 5% dioxane , VAPOR DIFFUSION, HANGING DROP, temperature 291K |