4O5R
Crystal structure of Alkylhydroperoxide Reductase subunit C from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-25 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 137.349, 137.349, 147.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.740 - 3.330 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3emp |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.696 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 147.450 | 3.510 |
| High resolution limit [Å] | 3.330 | 3.330 |
| Rmerge | 0.484 | |
| Number of reflections | 23735 | |
| <I/σ(I)> | 10.2 | 1.5 |
| Completeness [%] | 99.2 | 99.7 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.8M ammonium sulfate, 100mM MES, pH 6.5, 5% dioxane , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
