4O5P
Crystal structure of an uncharacterized protein from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-25 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 |
Unit cell lengths | 61.603, 81.078, 92.145 |
Unit cell angles | 97.83, 89.96, 90.01 |
Refinement procedure
Resolution | 28.298 - 2.001 |
R-factor | 0.2258 |
Rwork | 0.226 |
R-free | 0.26930 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.199 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.066 | 0.212 |
Number of reflections | 109609 | |
<I/σ(I)> | 24.6 | 4.3 |
Completeness [%] | 100.0 | 92.3 |
Redundancy | 3.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.04M citric acid, 0.1M Bis-Tris propane, 17% pEG 3350 , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |